NDLI: Seawater induces apoptosis in alveolar epithelial cells via the Fas/FasL-mediated pathway

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Seawater induces apoptosis in alveolar epithelial cells via the Fas/FasL-mediated pathway

Content Provider	Semantic Scholar
Author	Han, Feng Luo, Ying Li, Yanyan Liu, Zhongyang Li, Zhichao
Copyright Year	2012
Abstract	Our previous study showed that seawater can cause lung tissue cell apoptosis; in the present study, the immunohistochemistry and Western blot analysis results demonstrated that Fas, FasL, and cleaved caspase-8 and caspase-3 were up-regulated in the rat lungs exposed to seawater. We found that seawater-induced human lung alveolar epithelial A549 cell apoptosis was concentration and time dependent. Moreover, seawater increased the expression of Fas, FasL, and cleaved caspase-8 and caspase-3 in A549 cells. The incubation of A549 cells in the presence of FasL-neutralising antibody (NOK-2) or caspase-8 inhibitor (Z-IETD-FMK) resulted in a decrease of seawater-induced cell apoptosis. NOK-2 inhibited Fas/FasL interaction and reduced the cleavage of caspase-8 and caspase-3, and Z-IETD-FMK blocked caspase-8 and caspase-3 activation. Seawater similarly produced a significant increase in rat alveolar type II cell apoptosis and expression of Fas and cleaved caspase-8. In summary, the Fas/FasL pathway involved in alveolar epithelial cell (AEC) apoptosis could be important in the pathogenesis of seawater-induced acute lung injury (SW-ALI).
Starting Page	71
Ending Page	80
Page Count	10
File Format	PDF HTM / HTML
DOI	10.1016/j.resp.2012.05.012
PubMed reference number	22609371
Journal	Medline
Volume Number	182
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S1569904812001085
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S1569904812001085?dgcid=api_sd_search-api-endpoint
Journal	Respiratory Physiology & Neurobiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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