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The Interaction of Ethidium Bromide with Nucleic Acids
| Content Provider | Semantic Scholar |
|---|---|
| Author | Martz, William Warren |
| Copyright Year | 1971 |
| Abstract | The Interaction of Ethidium Bromide with Nucleic Acids Ethidium bromide (EB) is a phencnthridinium derivative. which interferes with nucleic acid metabolism in a variety of organisms apparently by forming a strong primary complex in which the planar phenanthridinium ring system is intercalated between adjacent base pairs of the DNA double heJ.i:>(.: Primary binding occurs up to a bound EB to DNA nucleotide ratio (r) of 0.20-0.25. At higher ratios a weaker. binding process occurs. The interaction of EB with DNA results in the induction of circular dichroic (CD) bands throughout the spectral region from 220 nm to 550 nm. One positive band centered near 307 nm reflects primary binding, whereas a negative ' . /, " band centered near 500 nm appears to reflect both primary and secondary binding. The molar circular dichroism (Ei~ER~~ at 307 nm, calculated on the basis of bound EB, incre~HH~s · :I': '•."' from zero at r. = O to +25. O at r = 0. 30, corresponding l.~ the saiuration of primary binding sites. The increase in EL-~R with increasing r indicates that the CD b~pd at 307 nm result,s from nearest-neighbor interactions between EB molecules bound to adja'cent primary binding sites. In the EBice quenched heat den~tured DNA complex, E1-ER is +12.89 at r = 0.10 compared to an E1-ER of +7.50 for EB-native DNA at |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ecommons.luc.edu/cgi/viewcontent.cgi?article=2148&context=luc_diss |
| Alternate Webpage(s) | https://ecommons.luc.edu/cgi/viewcontent.cgi?article=2148&context=luc_diss&httpsredir=1&referer= |
| Alternate Webpage(s) | https://ecommons.luc.edu/cgi/viewcontent.cgi?article=2148&context=luc_diss |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
