NDLI: Direct Facilitatory Role of Paragigantocellularis Neurons in Opiate Withdrawal-Induced Hyperactivity of Rat Locus Coeruleus Neurons: An In Vitro Study

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Direct Facilitatory Role of Paragigantocellularis Neurons in Opiate Withdrawal-Induced Hyperactivity of Rat Locus Coeruleus Neurons: An In Vitro Study

Content Provider	Semantic Scholar
Author	Kaeidi, Ayat Azizi, Hossein Javan, Mohammad Soleimani, Seyed Mohammad Ahmadi Fathollahi, Yaghoub Semnanian, Saeed
Copyright Year	2015
Abstract	Studies have shown that following opiate withdrawal, the spontaneous discharge rate of locus coeruleus (LC) neurons remarkably increases. Combination of intrinsic mechanisms with extrinsic excitatory modulations mediates the withdrawal-induced hyperactivity of LC neurons. The nucleus paragigantocellularis (PGi) provides the main excitatory inputs to LC and plays a pivotal role in opiate withdrawal. In the present study the direct facilitatory role of PGi on opiate withdrawal-induced hyperactivity of LC neurons was investigated using a newly developed brain slice, containing both LC and PGi. HRP retrograde neuronal tracing was used to verify the existence of both LC and PGi neurons in the developed slice. The spontaneous discharge rate (SDR), resting membrane potential (RMP) and spontaneous excitatory post-synaptic currents (sEPSCs) were recorded in LC neurons using whole cell patch clamp recording. Results showed that the net SDR and the net RMP of LC neurons in slices containing both LC and PGi neurons are significantly higher than slices lacking intact (uncut) PGi inputs. Also, the frequency of sEPSCs in those LC neurons receiving PGi inputs significantly increased compared to the slices containing no intact PGi inputs. Altogether, our results propose that increase in PGi-mediated excitatory transmission might facilitate the opiate withdrawal-induced hyperactivity of LC neurons.
File Format	PDF HTM / HTML
DOI	10.1371/journal.pone.0134873
PubMed reference number	26230639
Journal	Medline
Volume Number	10
Alternate Webpage(s)	https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0134873&type=printable
Alternate Webpage(s)	http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0134873.PDF
Journal	PloS one
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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