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Dna Sequencing in Both Directions
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gwangheon |
| Copyright Year | 2005 |
| Abstract | A DNA sequencing method has been developed whereby DNA that has been cloned in a single-stranded bacteriophage vector can be sequenced from both ends. The method involves f i r s t making a minus-strand sense template from a single-stranded insert in the vector MJ3mp2 using a flanking primer~ and then sequencing the synthesized template using the d i d e o x y n u c l e o t i d e termination method (Sanger e t a l . , 1977~ 1980) with a second primer. Special conditions are descr ibed under which the f i r s t primer is easily removed after making the templat% and sequencing in the opposi te d i r ec t ion can be done in the normal way (Sanger e t a l . , 1980) without separa t ing the double s t rands . This method renders it possible to read up to twice the amount of sequence data from a long insert and also to check short inserts by producing complementary sequence patterns. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bioscirep.org/content/ppbioscirep/1/3/243.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
