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Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Zhongliang Li, Qiang Hua Li, Qiufen Du, Simiao Zhou, Yongquan Lv, Chunming Zhao, Yan Wang, Yongjun Zhang, Ning |
| Copyright Year | 2014 |
| Abstract | Qi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP. We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol. The correlation coefficients of all calibration curves were greater than 0.99. Recoveries measured at three concentration levels varied from 95.43% to 102.35%. Relative standard deviations of intra- and inter-day precisions were less than 4.45%. After successfully validating our method, we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components. |
| Starting Page | 384 |
| Ending Page | 393 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.apsb.2014.05.003 |
| PubMed reference number | 26579408 |
| Journal | Medline |
| Volume Number | 4 |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/81138913.pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/04/5e/main.PMC4629094.pdf |
| Journal | Acta pharmaceutica Sinica. B |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
