NDLI: Utilization of cellulose microfiber from pineapple leaf as lipase immobilization support for highly retained activity, ease of separation and reusability

Please wait, while we are loading the content...

Utilization of cellulose microfiber from pineapple leaf as lipase immobilization support for highly retained activity, ease of separation and reusability

Content Provider	Semantic Scholar
Author	Pradubsang, Thitinun Inprakhon, Pranee Patikarnmonthon, Nisa Amornsakchai, Taweechai
Copyright Year	2018
Abstract	We investigate the use of cellulose microfibre from pineapple leaf, referred to as pineapple leaf microfibre (PALMF), as a support for the immobilization of lipase AY from Candida rugosa. PALMF was produced from pineapple leaf waste with a very fine diameter of about 3 μm. It disperses very well in aqueous media and can easily be filtered out. PALMF in the forms of loose fibre and of mat were used. Different amounts of enzyme were immobilized on these PALMFs and tested for their hydrolytic activity. The reusability of the immobilized enzyme was also studied. The results showed that, for the selected amount of lipase used, enzyme loading and PALMF form affect the hydrolytic activity of the enzyme. Immobilized enzymes display slightly lower hydrolytic activity than the free enzyme. The activity decreases when the enzyme loading (on PALMF) is increased. Lipase AY immobilized with optimum quantity (1.37 mg/g of PALMF support) displayed a 12% drop in activity from that of free enzyme. The crowding of enzyme molecules on the PALMF surface caused steric hindrance and reduced accessibility to the active site of the enzyme. So with the suitable amount of immobilized enzyme on PALMF, reasonable hydrolytic activity together with easy separation and reusability can be obtained. This method could aid significantly the use of enzyme in industrial processes.
Starting Page	226
Ending Page	226
Page Count	1
File Format	PDF HTM / HTML
DOI	10.2306/scienceasia1513-1874.2018.44.226
Volume Number	44
Alternate Webpage(s)	http://scienceasia.org/2018.44.n4/scias44_227.pdf
Alternate Webpage(s)	https://doi.org/10.2306/scienceasia1513-1874.2018.44.226
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in