NDLI: Stability and structural features of DNA intercalation with ethidium bromide , acridine orange and methylene blue

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Stability and structural features of DNA intercalation with ethidium bromide , acridine orange and methylene blue

Content Provider	Semantic Scholar
Author	Nafisi, Shohreh Saboury, Ali Akbar Keramat, Nahid Tajmir-Riahi, Heidar-Ali
Copyright Year	2006
Abstract	Ethidium bromide (EB), acridine orange (AO), methylene blue (MB) and other fluorescent compounds are often used to probe DNA structure in drug–DNA and protein–DNA interactions. They bind nucleic acids via intercalative mode and cause major changes to DNA and RNA structures. The aim of this study was to examine the stability and structural features of calf-thymus DNA complexes with EB, AO and MB in aqueous solution, using constant DNA concentration (12.5 mM) and various pigment/DNA(P) ratios of 1/40, 1/20, 1/ 10, 1/4 and 1/2. FTIR, UV–visible spectroscopy and isothermal titration calorimetry (ITC) are used to determine the ligand intercalation and external binding modes, the binding constant and the stability of pigment–DNA complexes in aqueous solution. Structural analysis showed major intercalation of EB, AO and MB into polynucleotides G–C and A–T base pairs with minor external binding and overall binding constants of KEB = 6.58 · 10 M , KAO = 2.69 · 10 M 1 and KMB = 2.13 ·10 M . The overall binding constants estimated by UV– visible spectroscopy are consistent with thermodynamic data obtained by ITC, which showed DH(EB) = 13.58 kJ/mol, DH(AO) = 14.63 kJ/mol and DH(MB) = 13.87 kJ/mol with dissociation constants of KEB = 15 lM, KAO = 36 lM and KMB = 46 lM. 2006 Elsevier B.V. All rights reserved.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://nathan.instras.com/MyDocsDB/doc-534.pdf
Language	English
Access Restriction	Open
Subject Keyword	Acridine Orange Acridines Ambient occlusion Base Pairing Calorimetry DNA Intercalation DUOXA1 gene Ethidium Bromide Interaction Ligands Methylene blue Nucleic Acids Pigment Polynucleotides RNA Remanence Skin Test End-Point Titration Spectroscopy, Fourier Transform Infrared Structural analysis Thermodynamics Thymic Tissue Titration Method carbene kilojoule (kJ)
Content Type	Text
Resource Type	Article

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