Anti-neuroinflammatory activity of nobiletin on suppression of microglial activation.

Content Provider	Semantic Scholar
Author	Cui, Yanji Wu, Jinji Jung, Sung-Cherl Park, Deok-Bae Maeng, Young-Hee Yun, Jeong Kim, Soon-Jong Kim, Sang Jeong Eun, Su-Yong
Copyright Year	2010
Abstract	A growing body of evidence suggests that nobiletin (5,6,7,8,3',4'-hexamethoxy flavone) from the peel of citrus fruits, enhances the damaged cognitive function in disease animal models. However, the neuroprotective mechanism has not been clearly elucidated. Since nobiletin has shown anti-inflammatory effects in several tissues, we investigated whether nobiletin suppresses excessive microglial activation implicated in neurotoxicity in lipopolysaccharide (LPS)-stimulated BV-2 microglia cell culture models. Release of nitric oxide (NO), the major inflammatory mediator in microglia, was markedly suppressed in a dose-dependent manner following nobiletin treatment (1-50 µM) in LPS-stimulated BV-2 microglia cells. The inhibitory effect of nobiletin was similar to that of minocycline, a well-known microglial inactivator. Nobiletin significantly inhibited the release of the pro-inflammatory cytokine tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β). LPS-induced phosphorylations of extracellular signal-regulated kinase (ERK), c-Jun NH(2)-terminal kinase (JNK), and p38 mitogen-activated protein kinases (MAPKs) were also significantly inhibited by nobiletin treatment. In addition, nobiletin markedly inhibited the LPS-induced pro-inflammatory transcription factor nuclear factor κB (NF-κB) signaling pathway by suppressing nuclear NF-κB translocation from the cytoplasm and subsequent expression of NF-κB in the nucleus. Taken together, these results may contribute to further exploration of the therapeutic potential and molecular mechanism of nobiletin in relation to neuroinflammation and neurodegenerative diseases.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/bpb/33/11/33_11_1814/_pdf/-char/ja
PubMed reference number	21048305v1
Volume Number	33
Issue Number	11
Journal	Biological & pharmaceutical bulletin
Language	English
Access Restriction	Open
Subject Keyword	Animal Diseases Animal Model Anti-Inflammatory Agents Body tissue Cell Culture Techniques Cell Nucleus Citrus plant Fruit Lipopolysaccharides Mediator brand of benfluorex hydrochloride Microglia Mitogen-Activated Protein Kinases Mitogens Neurodegenerative Disorders Neurogenic Inflammation Neurotoxicity Syndromes Nitric Oxide Phosphorylation TRANSCRIPTION FACTOR cytokine flavone mitogen-activated protein kinase p38 nobiletin tumor necrosis
Content Type	Text
Resource Type	Article