Loading...
Please wait, while we are loading the content...
Similar Documents
Direct Electrochemistry of Glucose Oxidase Immobilized on a Hydroxyl Fullerenes- Tio2 Nanocomposite Modified Glassy Carbon Electrode
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Xiao-Yu Xiao, Bao-Lin Akbar, Moosavi-Movahedi Ali |
| Copyright Year | 2017 |
| Abstract | By immobilizing glucose oxidase (GOD) on a nanocomposite containing hydroxyl fullerenes ( 60 C OH ) and 2 TiO nanoparticles, a new type biosensor was prepared for glucose detection. The cyclic voltammograms (CVs) of GOD on the nanocomposite modified glassy carbon electrode (GCE) was quasi-reversible. The heterogeneous electron transfer constant (ks) and the formal potential (Eó) of the Chi/GOD/ 2 60 C OH TiO /GCE were 3.0 s −1 and -0.248V, respectively. The Chi/GOD/ 2 60 C OH TiO /GCE responsed linearly to glucose from 0.05 to 0.85 mM. The apparent Michaelis–Menten constant ( app m K ) and detection limit of the Chi/GOD/ 2 60 C OH TiO /GCE were 1.17 mM and 0.05 mM, respectively. Introduction Glucose oxidase (GOD) is a glycoprotein with a relative rigid structure. Its molecular weight is around 150–180 kDa. In the present of GOD, glucose reacts with oxygen to produce gluconic acid and hydrogen peroxide [1]. It is usually difficult to observe the direct electron exchange between GOD and electrode surface, because the FAD groups are buried deeply inside the polypeptide chains of relatively large GOD molecules. Direct electron transfer of GOD on various types of modified electrodes has been investigated. And nanoparticles, such as Ag, Au, MnO2, Fe3O4 or TiO2, and carbon nanotubes (CNTs) have also been served in electrochemical study of redox proteins [2-12]. Among them, the combination of several kinds of nano-materials may bring together their unique properties and generate a new nanocomposite with superior characteristics [13-15]. In this report, we presented a novel GOD-Hydroxyl fullerenes-TiO2 nano composite immobilized glassy carbon electrode (GCE). The electrode was also covered with a layer of chitosan (Chi) film to improve its stability. The hydroxyl fullerenes (C60-OH) may form specific noncovalent complexes with a protein and help to protect the protein [16-17]. 331 This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/). Copyright © 2017, the Authors. Published by Atlantis Press. 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017) Advances in Biological Sciences Research (ABSR), volume 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.2991/bbe-17.2017.53 |
| Alternate Webpage(s) | https://download.atlantis-press.com/article/25879971.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
