The absence of a major Ca2+ signaling pathway in GABAergic neurons of the hippocampus.

Content Provider	Semantic Scholar
Author	Sík, Attila Hájos, Norbert Gulácsi, Alexandra Módy, István
Copyright Year	1998
Abstract	The Ca2+/calmodulin-dependent protein phosphatase 2B or calcineurin (CN) participates in several Ca2+-dependent signal transduction cascades and, thus, contributes to the short and long term regulation of neuronal excitability. By using a specific antibody to CN, we demonstrate its absence from hippocampal interneurons and illustrate a physiological consequence of such CN deficiency. Consistent with the lack of CN in interneurons as detected by immunocytochemistry, the CN inhibitors FK-506 or okadaic acid significantly prolonged N-methyl-D-aspartate channel openings recorded in the cell-attached mode in hippocampal principal cells but not those recorded in interneurons. Interneurons were also devoid of Ca2+/calmodulin-dependent protein kinase IIalpha, yet many of their nuclei contained the cyclic AMP-responsive element binding protein. On the basis of the CN and Ca2+/calmodulin-dependent protein kinase IIalpha deficiency of interneurons, entirely different biochemical mechanisms are expected to govern Ca2+-dependent neuronal plasticity in interneurons versus principal cells.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/95/6/3245.full.pdf
PubMed reference number	9501248v1
Volume Number	95
Issue Number	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Aspartic Acid Calcineurin Calcium ion Contain (action) Cyclic AMP Dopaminergic Neurons FK-506 Immunocytochemistry Interneurons N-Methylaspartate Neuronal Plasticity Okadaic Acid Protein Kinases Protein phosphatase Signal Transduction Pathways
Content Type	Text
Resource Type	Article