NDLI: Purification and identification of a novel antifungal protein secreted by Penicillium citrinum from the Southwest Indian Ocean.

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Purification and identification of a novel antifungal protein secreted by Penicillium citrinum from the Southwest Indian Ocean.

Content Provider	Semantic Scholar
Author	Wen, Chao Guo, Wenbin Chen, Xinhua
Copyright Year	2014
Abstract	A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 culture supernatants with ammonium sulfate and ion-exchange chromatography, an antifungal protein (PcPAF) was purified. The N-terminal amino acid sequence analysis showed that PcPAF might be an unknown antifungal protein. PcPAF displayed antifungal activity against Trichoderma viride, Fusarium oxysporum, Paecilomyces variotii, and Alternaria longipes at minimum inhibitory concentrations of 1.52, 6.08, 3.04, and 6.08 µg/disc, respectively. PcPAF possessed high thermostability and had a certain extent of protease and metal ion resistance. The results suggested that PcPAF may represent a novel antifungal protein with potential application in controlling plant pathogenic fungal infection.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jmb.or.kr/submission/Journal/024/JMB024-10-05_FDOC_1.pdf
PubMed reference number	24931500v1
Volume Number	24
Issue Number	10
Journal	Journal of microbiology and biotechnology
Language	English
Access Restriction	Open
Subject Keyword	Alternaria (fungus) Alternaria longipes Amino Acid Sequence Amino Acids Ammonium Sulfate Antifungal Agents Byssochlamys spectabilis Endopeptidases Fungi Fusarium oxysporum Ions Iontophoresis Mycoses Penicillium Sediment Sequence Analysis Supernatant Trichechus manatus Trichoderma viride
Content Type	Text
Resource Type	Article

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