Loading...
Please wait, while we are loading the content...
Similar Documents
Toll-like receptor 4 modulates myocardial ischaemia-reperfusion injury: Role of matrix metalloproteinases.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Stapel, Heidi Kim, Se-Chan Osterkamp, Steffen Knuefermann, Pascal Hoeft, Andreas Meyer, Rainer Grohé, Christian Baumgarten, Georg |
| Copyright Year | 2006 |
| Abstract | UNLABELLED Toll-like receptor 4 (TLR4) mediates innate immune responses following endotoxemia and myocardial ischaemia-reperfusion (I/R) injury. Pre-treatment with the major TLR4 ligand lipopolysaccharide (LPS) reduces infarct size. Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) play a crucial role in endotoxemia possibly also determining I/R injury. AIMS We investigated the influence of TLR4 on infarct size and assessed the influence of MMP and TIMP regulation on I/R injury. METHODS Left anterior descending artery (LAD) occlusion was performed on wild-type (C3H/HeN) and TLR4-deficient (C3H/HeJ) mice. Animals were stimulated with LPS (1 mg/kg) or PBS 16 h ahead of 60 min LAD ligation. After 24 h of reperfusion, triphenyltetrazolium chloride staining was performed and infarct size was measured by planimetry. MMP- and TIMP-mRNA expression were determined by RPA after 3 h of reperfusion. MMP zymographic activity was monitored 6 h after occlusion. RESULTS TLR4-deficient mice and LPS-treated wild-type mice showed significantly reduced infarct areas. LPS-stimulation significantly increased the overall MMP/TIMP mRNA expression ratio due to elevated MMP-3, -8, -9, and TIMP-1 in wild-type mice. I/R overall reduced the MMP/TIMP ratio due to increased MMP-1, TIMP-1, and -3 mRNA expression. CONCLUSIONS LPS pre-treatment and TLR4-deficiency led to a decreased infarct size. However, infarct area and MMP/TIMP ratio were not correlated. This means that in TLR4-deficient mice MMP/TIMP ratios are not determining the infarct size. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.ejheart.2006.03.005 |
| Alternate Webpage(s) | http://eurjhf.oxfordjournals.org/content/8/7/665.full.pdf |
| PubMed reference number | 16829192 |
| Alternate Webpage(s) | https://doi.org/10.1016/j.ejheart.2006.03.005 |
| Volume Number | 8 |
| Issue Number | 7 |
| Journal | European journal of heart failure |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
