NDLI: Disposable Labtube Cartridges for Automated Protein Purification in Standard Lab Centrifuges

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Disposable Labtube Cartridges for Automated Protein Purification in Standard Lab Centrifuges

Content Provider	Semantic Scholar
Author	Kloke, Arne Niekrawietz, S. Bernhardt, Jeff Kneusel, Richard Schemel, Karin Patricia Ritzel, Jonas Bohn Stetten, Felix Von Zengerle, Roland Paust, Nils
Copyright Year	2013
Abstract	The protein purification process is often the bottleneck for the efficient production of a large number of different proteins. Automation of these procedures is often the crux of the matter and is frequently a trade-off between efficiency and cost. Using our novel disposable LabTube cartridges we demonstrate how the process of His-tagged protein purification can be automated in standard laboratory centrifuges. LabTube cartridges include prestored reagents which are sequentially applied to a Ni-NTA purification matrix by an integrated ballpen mechanism actuated by acceleration changes of the centrifuge. Fully automated runs demonstrated similar yield and purity compared to manual purifications with sample addition as the only manual handling step. Thus, the user is available for parallel tasks during 95 % of the overall process time (33 min). In contrast manual processing requires the user to be present for 18 minutes out of the 33 minute overall process time. Since LabTube automation requires no investment in a special lab automation device, this platform lowers the market entry barrier for lab automation.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.imtek.de/data/lehrstuehle/app/dokumente/conferences-pdf/conferences-2013/Kloke-disposable-labtube-cartridges
Alternate Webpage(s)	http://www.rsc.org/images/loc/2013/PDFs/Papers/545_0643.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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