NDLI: Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA.

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Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA.

Content Provider	Semantic Scholar
Author	Ando, Hideki Okamoto, Hitoshi
Copyright Year	2003
Abstract	We previously reported mRNA caging technology as a novel and simple technique for photo-mediated temporal and spatial control of gene activation in zebrafish embryos and as an alternative to the 'gene knockdown' approach using antisense morpholino oligonucleotides. The caging reagent used is 6-bromo-4-diazomethyl-7-hydroxycoumarin (Bhc-diazo), which forms a covalent bond with the phosphate moiety of the sugar-phosphate backbone of RNA. Mainly because of the reduced solubility of caged mRNA in aqueous solutions, special care in handling is needed. The Bhc-diazo group binds to the phosphate moieties of RNA and abolishes the translational activity of the latter. The translational activity of Bhc-caged mRNA is restored by photolysis/uncaging when exposed to long-wave UV light (350 approximately 365 nm). In this paper we describe the technique and detailed procedures for spatially and temporally controlled induction of gene expression in zebrafish embryos.
Starting Page	25
Ending Page	31
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1023/B:MICS.0000006846.13226.38
PubMed reference number	14739584
Journal	Medline
Volume Number	25
Issue Number	1-2
Alternate Webpage(s)	http://www.sorenandersen.org/MICS/fAndoOkamoto.pdf
Alternate Webpage(s)	https://doi.org/10.1023/B%3AMICS.0000006846.13226.38
Journal	Methods in cell science : an official journal of the Society for In Vitro Biology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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