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Bioavailability of Soil Particle-bound Copper to lux -marked, Copper- specific Pseudomonas fluorescens Biosensor
| Content Provider | Semantic Scholar |
|---|---|
| Author | Koefoed, Kristian Holm, Peter E. Nybroe, Ole |
| Copyright Year | 2015 |
| Abstract | INTRODUCTION The measurement of metal bioavailability to soil dwelling organisms remains a challenge. Usually, only the soluble fraction of metals is considered bioavailable and thus able to exert a physiological response, but this has rarely been tested experimentally. Interestingly however, it has very recently been reported that a significant fraction of particle-bound metals such as cadmium and lead are bioavailable to specific bacterial whole-cell biosensors in soil-water mixtures (Ivask et al. 2004). Hence, up to 115-times more Cd and 40-times more Pb proved bioavailable to sensor bacteria incubated in soil suspensions rather than in aqueous soil extracts (Ivask et al. 2004). These somewhat surprising results highlight the need for further research in this field. The aim of the present work was to develop a solid phase-contact bioassay based on a Cuspecific, luxAB-marked Pseudomonas fluorescens reporter bacterium allowing for the direct interaction between sensor cells and particle-bound Cu in soil and to evaluate the bioavailability of particle-bound Cu under different experimental conditions. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://plen.ku.dk/english/employees/?pure=files/8649610/BrandtKKetal_1_.2005_ICOBTE8.pdf |
| Alternate Webpage(s) | http://static-curis.ku.dk/portal/files/8649610/BrandtKKetal_1_.2005_ICOBTE8.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
