Loading...
Please wait, while we are loading the content...
Similar Documents
Confocal Laser Endomicroscopy for the Morphometric Evaluation of Microvessels in Human Colorectal Cancer Using Targeted Anti-CD 31 Antibodies
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cârţână, Tatiana Saftoiu, Adrian Gruionu, Lucian Gheorghe Gheonea, Dan Ionuț Pirici, Daniel Georgescu, Claudia Valentina Ciocâlteu, Adriana Mihaela Gruionu, Gabriel |
| Copyright Year | 2012 |
| Abstract | Introduction: Numerous anti-angiogenic agents are currently developed to limit tumor growth and metastasis. While these drugs offer hope for cancer patients, their transient effect on tumor vasculature is difficult to assess in clinical settings. Confocal laser endomicroscopy (CLE) is a novel endoscopic imaging technology that enables histological examination of the gastrointestinal mucosa. The aim of the present study was to evaluate the feasibility of using CLE to image the vascular network in fresh biopsies of human colorectal tissue. For this purpose we have imaged normal and malignant biopsy tissue samples and compared the vascular network parameters obtained with CLE with established histopathology techniques. Materials and Methods: Fresh non-fixed biopsy samples of both normal and malignant colorectal mucosa were stained with fluorescently labeled anti-CD31 antibodies and imaged by CLE using a dedicated endomicroscopy system. Corresponding biopsy samples underwent immunohistochemical staining for CD31, assessing the microvessel density (MVD) and vascular areas for comparison with CLE data, which were measured offline using specific software. Results: The vessels were imaged by CLE in both normal and tumor samples. The average diameter of normal vessels was 8.560.9 mm whereas in tumor samples it was 13.560.7 mm (p = 0.0049). Vascular density was 188.7624.9 vessels/mm in the normal tissue vs. 242.4616.1 vessels/mm in the colorectal cancer samples (p = 0.1201). In the immunohistochemistry samples, the MVD was 211.2642.9/mm and 351.3639.6/mm for normal and malignant mucosa, respectively. The vascular area was 2.960.5% of total tissue area for the normal mucosa and 8.562.1% for primary colorectal cancer tissue. Conclusion: Selective imaging of blood vessels with CLE is feasible in normal and tumor colorectal tissue by using fluorescently labeled antibodies targeted against an endothelial marker. The method could be translated into the clinical setting for monitoring of anti-angiogenic therapy. Citation: Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, et al. (2012) Confocal Laser Endomicroscopy for the Morphometric Evaluation of Microvessels in Human Colorectal Cancer Using Targeted Anti-CD31 Antibodies. PLoS ONE 7(12): e52815. doi:10.1371/journal.pone.0052815 Editor: Anthony W.I. Lo, The Chinese University of Hong Kong, Hong Kong Received September 10, 2012; Accepted November 21, 2012; Published December 28, 2012 Copyright: 2012 Cârţână et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported from the research grant entitled ''Clinical and Biomathematical Modeling of Vascular Changes Following Anti-Angiogenic Therapy in Advanced Colorectal Carcinoma'', funded by the National Research Council (CNCS), Romania, contract number PN-II-ID-PCE-2011-3-0664. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: adrian.saftoiu@webmail.umfcv.ro |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://dash.harvard.edu/bitstream/handle/1/11181208/3532115.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
