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Nociceptin is a potent inhibitor of N-type Ca2+ channels in rat sympathetic ganglion neurons
| Content Provider | Semantic Scholar |
|---|---|
| Author | Larsson, Kim P. Olsen, Uffe Bang Hansen, Anker Jon |
| Copyright Year | 2000 |
| Abstract | Nociceptin, an endogenous agonist of the opioid receptor-like(1) (ORL(1)) receptor, is implicated in a wide range of physiological functions including cardiovascular control. However, the effect of nociceptin on peripheral sympathetic ganglion neurons has not been studied. Whole-cell voltage clamp was used to study Ca(2+) currents on freshly dissociated sympathetic superior cervical ganglion neurons from juvenile rats. Nociceptin (1 microM) caused a fast inhibition of the peak currents by 69+/-3% in all neurons. Strong positive prepulses counteracted the inhibition of the peak current by 64% and no effect of nociceptin was observed when the cells were pre-incubated with Pertussis toxin. The inhibition was reversible and dose-dependent with an EC(50) of 508+/-50 pM. Blockade of N-type channels by 1 microM omega-conotoxin GVIA reduced the peak currents by 83+/-1% and abolished the action of nociceptin. Naloxone could not prevent the inhibition by nociceptin and [D-Ala(2), N-Me-Phe(4), Gly(5)-ol] enkephalin (DAMGO) only depressed a small proportion of the current in 1/7 neurons. These data suggests that nociceptin inhibits transmitter release from sympathetic neurons by a selective blockade of N-type channels, which may be of importance for its depressive effect on the cardiovascular system. |
| Starting Page | 121 |
| Ending Page | 124 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0304-3940(00)01640-2 |
| PubMed reference number | 11108996 |
| Journal | Medline |
| Volume Number | 296 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0304394000016402 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0304394000016402?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/S0304-3940%2800%2901640-2 |
| Journal | Neuroscience Letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
