NDLI: Continuous Flow Digital Loop-mediated Isothermal Amplification ( Lamp ) in Droplets

Content Provider	Semantic Scholar
Author	Rane, Tushar D. Chen, L. L. Zec, Helena C. Wang, Ti Ho
Copyright Year	2014
Abstract	Digital PCR is a newly emerging technology, very useful in precise nucleic acid quantification at single molecule level, even in the presence of large concentration of background DNA. However, the current commercially available instruments for digital PCR are limited in application due to a fragmented workflow requiring multiple instruments and low throughput. Here we propose a continuous flow digital loop-mediated isothermal amplification (LAMP) technique as an alternative to digital PCR. Successful application of this technique to digital nucleic acid detection demonstrates the potential of this technique to overcome all the issues associated with the digital PCR workflow.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.rsc.org/images/loc/2014/PDFs/Papers/538_0229.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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