NDLI: Facile regeneration through adventive/somatic embryogenesis from in vitro cultured immature leaf segments of elite varieties of sugarcane (Saccharum officinarum L)

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Facile regeneration through adventive/somatic embryogenesis from in vitro cultured immature leaf segments of elite varieties of sugarcane (Saccharum officinarum L)

Content Provider	Semantic Scholar
Author	Ali, Safdar Iqbql, Javed
Copyright Year	2010
Abstract	This research work was conducted to optimize protocol for in vitro regeneration through embryogenesis, of nine elite varieties of sugarcane. In all the nine varieties, somatic embryogenesis with and without an intervening callus phase was achieved under the influence of varying concentrations of 2,4-D ranging from 1.5 to 2.5mg -l . Embryo development was seen all over the cultured explants within four weeks of culture and the transfer of either adventive embryos or somatic embryos from embryo induction medium to embryo germination medium containing casein hydrolysate (500mgl -l ) with 2,4-D (1mgl -l ) or without growth regulators favoured embryo maturation thus resulting in plantlet regeneration within 6 weeks and all the regenerated plantlets grew normally in the greenhouse. Embryogenesis and regeneration was not significantly influenced by the variety. The technique for embryo induction involved culturing of leaf discs derived from immature leaf rolls, on medium containing 2,4-D. Plantlets were ready for hardening after 10-14 week. In a comparative study, transformation efficiency for the direct embryogenic morphogenic route was more than for indirect embryogenic morphogenic route. Due to fewer subcultures, the cost of production via direct somatic embryogenesis can be reduced per transgenic plant. This method could be useful to follow and exploit somaclonal variations as well as provide a target tissue for genetic transformation studies.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://biosoc.pk/wp-content/uploads/2013/02/7.sugarcane-Dr-Safdar.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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