Efficient trans-splicing of a yeast mitochondrial RNA group II intron implicates a strong 5' exon-intron interaction.

Content Provider	Semantic Scholar
Author	Jacquier, Alain Rosbash, Michael
Copyright Year	1986
Abstract	The reaction mechanism for self-splicing introns requires the existence of a 5' exon binding site on the intron. Experimental evidence is now presented consistent with the existence of such a binding site by demonstrating efficient and accurate trans-self-splicing of a yeast mitochondrial group II intron. Partial and complete trans-splicing reactions take place in the absence of branch formation, part of the usual pathway of nuclear splicing and group II self-splicing. In addition to indicating the existence of a 5' exon binding site on the intron, the results have mechanistic implications for group II self-splicing and perhaps for nuclear splicing as well.
File Format	PDF HTM / HTML
DOI	10.1126/science.2430332
Alternate Webpage(s)	http://www.bio.brandeis.edu/rosbash-lab/PublishedPapers/1982-1986/Jacquier-Science-1986.pdf
Alternate Webpage(s)	http://www.bio.brandeis.edu/rosbashlab/PublishedPapers/1982-1986/Jacquier-Science-1986.pdf
PubMed reference number	2430332
Alternate Webpage(s)	https://doi.org/10.1126/science.2430332
Journal	Medline
Volume Number	234
Issue Number	4780
Journal	Science
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article