NDLI: Development of an epitope-based competitive ELISA for the detection of antibodies against Tibetan peste des petits ruminants virus.

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Development of an epitope-based competitive ELISA for the detection of antibodies against Tibetan peste des petits ruminants virus.

Content Provider	Semantic Scholar
Author	Zhang, Guo-Rui Yu, Rui-song Zeng, Jiang-Yong Zhu, Yu-min Dong, Shi-juan Dunzhu, Luobu Zhu, Se Duoji, Ciren Lei, Zhihai Li, Zhen
Copyright Year	2013
Abstract	AIMS To develop an effective diagnostic kit, based on a competitive ELISA-based system (cELISA), for detecting serum antibody against peste des petits ruminants virus (PPRV). METHODS Epitope peptides of the nucleocapsid (N) protein of Tibetan PPRV were synthesized chemically and injected into rabbits to prepare hyperimmune antisera. Test sera were incubated simultaneously with hyperimmune antisera and added to the wells of ELISA plates coated previously with recombinant N protein. Horseradish peroxidase-conjugated goat anti-rabbit antibody was employed to detect the quantity of hyperimmune antisera combined with recombinant N protein. RESULTS A cELISA has been developed for monitoring PPRV infections with a cutoff value of 35. Relative sensitivity and specificity values of the epitope-based cELISA were 96.18 and 91.29%, respectively, when compared with a commercial cELISA kit in a test involving 1,039 serum samples. CONCLUSION We report an efficient method for preparing antibody suitable for incorporation into a cELISA that can be used routinely for the detection of PPRV antibodies in serum samples. The method eliminated the requirement for virus culture and monoclonal antibody preparation, reduced the biorisk posed by virus-dependent manipulations, and the performance of the resultant cELISA compared favorably with a commercially available cELISA kit.
Starting Page	165
Ending Page	178
Page Count	14
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.karger.com/Article/Pdf/341601
PubMed reference number	23052035v1
Alternate Webpage(s)	https://doi.org/10.1159/000341601
DOI	10.1159/000341601
Journal	Intervirology
Volume Number	56
Issue Number	1
Language	English
Access Restriction	Open
Subject Keyword	Enzyme-Linked Immunosorbent Assay Horseradish Peroxidase Immune Sera Immunostimulating conjugate (antigen) Mast/Stem Cell Growth Factor Receptor Kit, human Monoclonal Antibodies Murine sarcoma viruses Peste des petits ruminants Infection Peste-des-petits-ruminants virus Preparation Recombinants incubated viral nucleocapsid location
Content Type	Text
Resource Type	Article

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