NDLI: Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage.

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Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage.

Content Provider	Semantic Scholar
Author	Han, Kyu-Lee Lee, Jongweon Kim, Don-Soo Park, Soon-Jung Im, Kyung-Il Yong, Tai-Soon
Copyright Year	2006
Abstract	Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from 5% to 70%. Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.
Starting Page	15
Ending Page	20
Page Count	6
File Format	PDF HTM / HTML
DOI	10.3347/kjp.2006.44.1.15
PubMed reference number	16514277
Journal	Medline
Volume Number	44
Issue Number	1
Alternate Webpage(s)	https://ir.ymlib.yonsei.ac.kr/bitstream/22282913/109306/1/T200600454.pdf
Journal	The Korean journal of parasitology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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