Replication of duplex DNA of phage phi X174 reconstituted with purified enzymes.

Content Provider	Semantic Scholar
Author	Arai, Kiyoko Arai, Nobutaka Shlomai, Joseph Kornberg, Arthur
Copyright Year	1980
Abstract	Replication of the covalently closed duplex replicative form (RF) of phage phi X174 DNA has been achieved by coupling two known enzyme systems: (i) synthesis of viral strand circles (SS) from RF, and (ii) conversion of SS to nearly complete RF (RF II). In this coupled system, activated RF (gene A . RF II complex) was a more efficient template and generated as many as 10 RF II molecules per RF input, at a rate commensurate with SS synthesis. The 11 proteins required for the two component systems were all needed in the coupled RF duplication system; no new factors were required. Single-stranded DNA binding protein was needed for RF duplication at only 4% the level needed in its stoichiometric participation in SS synthesis. In addition to RF II, more complex replicative forms appeared late in the reaction, and their possible origin is discussed.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.77.6.3322
PubMed reference number	6447874
Journal	Medline
Volume Number	77
Issue Number	6
Alternate Webpage(s)	https://profiles.nlm.nih.gov/ps/access/WHBBKS.pdf
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article