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Efecto antifúngico del aceite esencial del clavo de olor (Syzygium aromaticum) sobre cepas de Cándida albicans: Estudio In vitro
| Content Provider | Semantic Scholar |
|---|---|
| Author | Andrade, Jácome Alejandra, Mayra |
| Copyright Year | 2019 |
| Abstract | Fuentes, mentions that oral candidiasis is caused by a fungus called Candida albicans, which is a normal component of the oral microflora, the etiology of this disease is both the lack of hygiene, predisposing factors, as well as the development of certain tolerance of this microorganism against various antimicrobial agents in routine use. This study evaluated the antifungal effect of the clove essential oil (Syzygium aromaticum) at different concentrations (5, 10 and 20%) and at different times (48, 72 and 96 hours) on strains of Candida albicans, by means of an experimental study (in vi-tro). The clove essential oil (Syzygium aromaticum) for the investigation, was obtained by means of the steam distillation technique, and diluted to concentrations of 5%, 10% and 20%, to determine the antifungal effect on 15 crops of Candida albicans seeded in Petri dishes with Sabouraud Agar, to each culture, three filter paper discs embedded with clove essential oil were placed at three different concentrations, as well as two other discs that corresponded to the positive control that was made with gluconate of 0.12% chlorhexidine and the negative control with physiological saline, they were incubated at a temperature of 37 ° C under aerobic conditions to later observe and measure the inhibition halos at 48, 72 and 96 hours of incubation, The results obtained were statistically evaluated and it was concluded that the clove essential oil at the different concentrations has an inhibitory effect on the Candida albicans strains, which decreases over time. Additionally, a physical-chemical study of the extract obtained was carried out in order to establish relationships between the inhibitory effect and the concentration of the active principle, (Eugenol), which was presented in a concentration of 91.38%. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.dspace.uce.edu.ec/bitstream/25000/18228/1/T-UCE-0015-ODO-121.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |