Loading...
Please wait, while we are loading the content...
Similar Documents
Adjuvant effect of a novel water-soluble polysaccharide isolated from the stem of Physalis alkekengi L. var. francheti (Mast.) Makino
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shang, Dongjie J. Zhang, Lili Han, Shuying Wang, Guiyun |
| Copyright Year | 2011 |
| Abstract | Water-soluble polysacchride (WSP) was isolated and purified from the mature stem ofPhysalis alkekengi L. var. francheti (Mast.) Makino, which is a traditional Chinese medicine herb. Gas chromatography (GC) analysis indicated that WSP was an acid heteropolysaccharide, which was composed of Rha, Ara, Gal, Glc and GalA in molar ratio of 2.6 : 1.0 : 6.5 : 3.5 : 2.4. More importantly, adjuvant activity of WSP was evaluated in ICR mice in present study. Male ICR mice were immunized subcutaneously with ovalbumin (OVA) alone, OVA/WSP, OVA/QuilA, and saline two times at 14-day interval. The mice were sacrificed two weeks after the last immunization to analyze antibody titers in serum. It was observed that WSP significantly enhanced OVA-specific antibody titers (IgG, IgG1, IgG2b) in serum when compared with OVA-injected mice (P<0.05 or P<0.01). Thus, WSP could be considered as a promising adjuvant eliciting both Th1 and Th2 responses to improve the efficacy of vaccine. Key words: Herbal medicine, Physalis alkekengi L., polysaccharide, adjuvant. |
| Starting Page | 3814 |
| Ending Page | 3818 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Volume Number | 5 |
| Alternate Webpage(s) | https://academicjournals.org/article/article1380708510_Shang%20et%20al.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
