Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation

Content Provider	Semantic Scholar
Author	Ab, Syarifah Darah, Ibrahim Omar, Ibrahim Che
Copyright Year	2009
Abstract	A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30oC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.
File Format	PDF HTM / HTML
DOI	10.5580/2779
Volume Number	9
Alternate Webpage(s)	https://print.ispub.com/api/0/ispub-article/13131
Alternate Webpage(s)	https://doi.org/10.5580/2779
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article