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Testosterone Regulates NUCB2 mRNA Expression in Male Mouse Hypothalamus and Pituitary Gland
| Content Provider | Semantic Scholar |
|---|---|
| Author | Seon, Sojeong Jeon, Daun Kim, Heejeong Chung, Yiwa Choi, Narae Yang, Hyunwon |
| Copyright Year | 2017 |
| Abstract | Nesfatin-1/NUCB2 is known to take part in the control of the appetite and energy metabolism. Recently, many reports have shown nesfatin-1/NUCB2 expression and function in various organs. We previously demonstrated that nesfatin-1/NUCB2 expression level is higher in the pituitary gland compared to other organs and its expression is regulated by 17β-estradiol and progesterone secreted from the ovary. However, currently no data exist on the expression of nesfatin-1/NUCB2 and its regulation mechanism in the pituitary of male mouse. Therefore, we examined whether nesfatin-1/NUCB2 is expressed in the male mouse pituitary and if its expression is regulated by testosterone. As a result of PCR and western blotting, we found that a large amount of nesfatin-1/NUCB2 was expressed in the pituitary and hypothalamus. The NUCB2 mRNA expression level in the pituitary was decreased after castration, but not in the hypothalamus. In addition, its mRNA expression level in the pituitary was increased after testosterone treatment in the castrated mice, whereas, the expression level in the hypothalamus was significantly decreased after the treatment with testosterone. The in vitro experiment to elucidate the direct effect of testosterone on NUCB2 mRNA expression showed that NUCB2 mRNA expression was significantly decreased with testosterone in cultured hypothalamus tissue, but increased with testosterone in cultured pituitary gland. The present study demonstrated that nesfatin-1/NUCB2 was highly expressed in the male mouse pituitary and was regulated by testosterone. This data suggests that reproductive-endocrine regulation through hypothalamus-pituitary-testis axis may contribute to NUCB2 mRNA expression in the mouse hypothalamus and pituitary gland. |
| Starting Page | 71 |
| Ending Page | 78 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/e8/dr-21-1-71.PMC5409212.pdf |
| PubMed reference number | 5409212 |
| Alternate Webpage(s) | https://doi.org/10.12717/DR.2017.21.1.071 |
| DOI | 10.12717/dr.2017.21.1.071 |
| Journal | Development & reproduction |
| Volume Number | 21 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Axis vertebra Castration Cell secretion Desire for food Energy Metabolism Hypothalamic structure In Vitro [Publication Type] NUCB2 gene Organ Ovary Posterior Pituitary Hormones Progesterone Reproduction Testosterone Western Blotting |
| Content Type | Text |
| Resource Type | Article |
