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A novel human organic anion transporting polypeptide localized to the basolateral hepatocyte membrane.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Penggang Nies, Anne T. Keppler, Deana |
| Copyright Year | 2000 |
| Abstract | We cloned and expressed a new organic anion transporting polypeptide (OATP), termed human OATP2, (OATP-C, LST-1; symbol SLC21A6), involved in the uptake of various lipophilic anions into human liver. The cDNA encoding OATP2 comprised 2073 base pairs, corresponding to a protein of 691 amino acids, which were 44% identical to the known human OATP. An antibody directed against the carboxy terminus localized OATP2 to the basolateral membrane of human hepatocytes. Northern blot analysis indicated a strong expression of OATP2 only in human liver. Transport mediated by recombinant OATP2 and its localization were studied in stably transfected Madin-Darby canine kidney strain II (MDCKII) and HEK293 cells. Confocal microscopy localized recombinant OATP2 protein to the lateral membrane of MDCKII cells. Substrates included 17beta-glucuronosyl estradiol, monoglucuronosyl bilirubin, dehydroepiandrosterone sulfate, and cholyltaurine. 17beta-Glucuronosyl estradiol was a preferred substrate, with a Michaelis-Menten constant value of 8.2 microM; its uptake was Na(+) independent and was inhibited by sulfobromophthalein, with a inhibition constant value of 44 nM. Our results indicate that OATP2 is important for the uptake of organic anions, including bilirubin conjugates and sulfobromophthalein, in human liver. |
| Starting Page | 1 |
| Ending Page | 13 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajpgi.physiology.org/content/ajpgi/278/1/G156.full.pdf |
| PubMed reference number | 10644574v1 |
| Volume Number | 278 |
| Issue Number | 1 |
| Journal | American journal of physiology. Gastrointestinal and liver physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Anion Exchange Protein 1, Erythrocyte Anions Base Pairing Bilirubin Carboxyl Group Clone Cells DNA, Complementary Dehydroepiandrosterone Sulfate Estradiol Hepatocyte Organic Anion Transport Polypeptides Recombinants Renal Tissue SLCO1B1 gene SLCO1B1 wt Allele Staphylococcal Protein A Sulfobromophthalein Taurocholic Acid Tissue membrane lipophilicity |
| Content Type | Text |
| Resource Type | Article |
