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Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Madhaiyan, Munusamy Roy, Joseph J. Kim, Soojin Weon, Hang-Yeon Kwon, Soon-Wo Ji, Lianghui |
| Copyright Year | 2013 |
| Abstract | A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392(T), was isolated from surface-sterilized root tissue of Jatropha curcas. The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0-8.0. The predominant ubiquinone was Q-10 and the major fatty acids were C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and C19 : 0 cyclo ω8c. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392(T) is affiliated with the order Rhizobiales within the class Alphaproteobacteria and is most closely related to Pleomorphomonas oryzae F-7(T) (98.8 % similarity) and Pleomorphomonas koreensis Y9(T) (98.3 % similarity). Analysis of partial nifH gene sequences also revealed a monophyletic lineage within the class Alphaproteobacteria, and strain R5-392(T) was most closely related to P. oryzae F-7(T) (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392(T) represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas diazotrophica sp. nov. is proposed. The type strain is R5-392(T) ( = KACC 16233(T) = DSM 25022(T)). |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/ijs.0.044461-0 |
| PubMed reference number | 23223815 |
| Journal | Medline |
| Volume Number | 63 |
| Part | 7 |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/63/7/2477_ijs044461.pdf?accname=guest&checksum=DBAA514640F33B72321C958E9E9D6308&expires=1539537759&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/63/7/2477_ijs044461.pdf?accname=guest&checksum=35125C5CBA984526B08D02445E1792A4&expires=1542969829&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/63/7/2477_ijs044461.pdf?accname=guest&checksum=3BD6F9BC529A34BFFAFD5DB636F7DF3D&expires=1541300831&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/ijsem/63/7/2477_ijs044461.pdf?accname=guest&checksum=7213150CAB60AE1FAF70ED3AE0B07D8E&expires=1549737007&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/ijsem/63/7/2477_ijs044461.pdf?accname=guest&checksum=F5FCA623F2CE7AD0DA14EAC8E293CA79&expires=1545117489&id=id |
| Alternate Webpage(s) | https://doi.org/10.1099/ijs.0.044461-0 |
| Journal | International journal of systematic and evolutionary microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
