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A low-cost, disposable card for rapid polymerase chain reaction.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jia, Guangyao Siegrist, Jonathan Deng, Chengwu Zoval, Jim V. Stewart, Gale Peytavi, Régis Huletsky, Ann Bergeron, Michel G. Madou, Marc J. |
| Copyright Year | 2007 |
| Abstract | A low-cost, disposable card for rapid polymerase chain reaction (PCR) was developed in this work. Commercially available, adhesive-coated aluminum foils and polypropylene films were laminated with structured polycarbonate films to form microreactors in a card format. Ice valves [1] were employed to seal the reaction chambers during thermal cycling and a Peltier-based thermal cycler was configured for rapid thermal cycling and ice valve actuation. Numerical modeling was conducted to optimize the design of the PCR reactor and investigate the thermal gradient in the reaction chamber in the direction of sample thickness. The PCR reactor was experimentally characterized by using thin foil thermocouples and validated by a successful amplification of 10 copy of E. coli tuf gene in 27 min. |
| Starting Page | 52 |
| Ending Page | 60 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.thesiegrists.com/Jons_Personal_Web_Site/Full_Papers_files/Colloids%20and%20surfaces%20B%20Biointerfaces%202007%20Jia.pdf |
| PubMed reference number | 17499979v1 |
| Volume Number | 58 |
| Issue Number | 1 |
| Journal | Colloids and surfaces. B, Biointerfaces |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
