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Low-cost, frequency-domain, fluorescence lifetime confocal microscopy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Booth, Martin Wilson, Tony |
| Copyright Year | 2004 |
| Abstract | We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime images of biological specimens from a single confocal scan. |
| Starting Page | 36 |
| Ending Page | 42 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.eng.ox.ac.uk/som/publications/som_2004_2.PDF |
| PubMed reference number | 15049866v1 |
| Volume Number | 214 |
| Part | 1 |
| Journal | Journal of microscopy |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Diode Device Component Fluorescence Lasers, Semiconductor Microscope Device Component Microscopy, Confocal Physical object Specimen |
| Content Type | Text |
| Resource Type | Article |