NDLI: Transcriptome Analysis of Leaves and Roots of Musa balbisiana var. 'Pisang Klutuk Wulung'

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Transcriptome Analysis of Leaves and Roots of Musa balbisiana var. 'Pisang Klutuk Wulung'

Content Provider	Semantic Scholar
Author	Santos, Candice Mello Romero Ferreira, Claudia Fortes Cassiano, Lizangela Pinheiro Catarina, Marly Coelho, Felipe De Macedo Martins, Natália Florencio Teixeira, Manoel Júnior, Souza
Copyright Year	2010
Abstract	Banana and plantain (Musa spp.) are perennial herbs belonging to the Musaceae family. Almost the totality of cultivated banana genotypes originated from natural intra- and inter-specific hybridization of two diploid species; Musa acuminata Colla and Musa balbisiana Colla; representing the A and B genomes, respectively. This study reports the construction and characterization of two cDNA libraries from the M. balbisiana var. ‘Pisang Klutuk Wulung’ (BB), also known as PKW. Roots and leaves from PKW hydroponics plants were used to construct these two cDNA libraries. Sequencing of cDNA clones, followed by trimming and CAP3 assembling of high quality ESTs resulted in 2,967 MbAES (Musa balbisiana Assembled EST Sequences), with 435 contigs and 2,532 singletons. Approximately 38% of the MbAES came out as “no hits” after Blastx search against several public databases. Assembled EST sequences were classified into 23 different categories according to putative protein functions (KOG), whereas the range of similarity was from 35 to 60%. The classification of the EST clusters into the Gene Ontology (GO) function classes showed a predominance of catalytic activity and binding as molecular function. Among the most populated contigs there were genes related to pathogenicity response, stress response and photosynthesis. A differential digital display between M. acuminata data already stored at the DATAMusa and M. balbisiana assembled EST sequences revealed 10 unknown genes exclusive to the B genome and nine exclusive to the A genome with statistically significant difference.
Starting Page	77
Ending Page	82
Page Count	6
File Format	PDF HTM / HTML
Volume Number	4
Alternate Webpage(s)	http://www.globalsciencebooks.info/Online/GSBOnline/images/2010/TFSB_4(SI1)/TFSB_4(SI1)77-82o.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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