NDLI: Variability of exhaled breath condensate leukotriene B4 and 8-isoprostane in COPD patients

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Variability of exhaled breath condensate leukotriene B4 and 8-isoprostane in COPD patients

Content Provider	Semantic Scholar
Author	Borrill, Zoë L. Starkey, Richard Singh, Sunita D.
Copyright Year	2007
Abstract	The reproducibility of exhaled breath condensate (EBC) mediators is not well documented in chronic obstructive pulmonary disease (COPD). This study assessed within assay (WA), within (WD) and between day (BD) reproducibility of EBC leukotriene B4 (LTB4) and 8-isoprostane. Three EBC samples were collected from 24 COPD patients separated by 1 h and 1 wk, to assess WD and BD reproducibility. WA reproducibility was assessed by sample analysis by enzyme immunoassay in triplicate. WA coefficient of variation for LTB4 and 8-isoprostane (18.2% and 29.2%, respectively) was lower than corresponding values for WD (47.7% and 65.3%, respectively) and BD (75.7% and 79.1%, respectively). Repeatability coefficient for 8-isoprostane and LTB4 assays were 18.6 pg/ml and 13.2 pg/ml, respectively. Group mean differences for WD and BD were small and statistically nonsignificant. Using the Bland Altman method, there were wide limits of agreement for WD (-51.6 to 47.2 for 8-isoprostane and -31.8 to 31.4 for LTB4) and BD reproducibility (-61.4 to 75.7 for 8-isoprostane and -29.3 to 38.6 for LTB4). This is the first study to fully report the variability of EBC 8-isoprostane and LTB4 in COPD. WA variability and group mean changes were small. However, we observed considerable WD and BD variability for these biomarkers.
Starting Page	71
Ending Page	76
Page Count	6
File Format	PDF HTM / HTML
PubMed reference number	18044068
Volume Number	2
Journal	International journal of chronic obstructive pulmonary disease
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/79/86/copd-2-71.PMC2692117.pdf
Language	English
Access Restriction	Open
Subject Keyword	8-isoprostaglandin F2alpha Breath Tests CD19 Antigens Chronic Obstructive Airway Disease Coefficient Enzyme Immunoassay Exhaled breath condensate (substance) Expiration, function Hepatolenticular Degeneration Immunoassay method Leukotriene B4 Leukotrienes Lung Diseases, Obstructive Lung diseases Nanogram per Liter Patients Triplicate
Content Type	Text
Resource Type	Article

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