NDLI: An efficient direct shoot regeneration from cotyledonary node explants of peanut (Arachis hypogaea L. CV. JL-24)

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An efficient direct shoot regeneration from cotyledonary node explants of peanut (Arachis hypogaea L. CV. JL-24)

Content Provider	Semantic Scholar
Author	Beena, M. R. Sravan Kumar Jami Srinivasan, Thangavelu Anuradha, T. Swathi Padmaja, G. Kirti, Pulugurtha Bharadwaja
Copyright Year	2005
Abstract	The shoot regeneration response from different explants, viz. cotyledon, cotyledonary node and petiolule, of the most popular Indian cultivar, JL-24 of peanut (Arachis hypogaea L.), was evaluated by culturing on Murashigeand Skoog medium containing B 5 vitamins supplemented with different growth regulators. Shoot bud initiation, proliferation and subsequent development into shoots were found to be superior from cotyledonary node explants in comparison to other explants. High frequency (69%) direct shoot regeneration from cotyledonary node was observed on MS medium with 4.0 mg I - 1 BAP and 0.2 mg I - 1 NAA. The shoots elongated upon transfer to MS medium with lower concentrations of BAP. Elongated shoots could be continuously harvested at 15 days interval for a period of 75 to 90 days. The regenerated shoots were rooted at a high frequency of 84.5% on MS medium containing 1.0 mg I - 1 NAA. The regenerated plantlets survived to maturity in greenhouse, flowered, set pods and seed normally. This protocol could be very efficiently used for transformation studies.
Starting Page	205
Ending Page	210
Page Count	6
File Format	PDF HTM / HTML
Volume Number	10
Alternate Webpage(s)	http://inorderbox.com/siteimages/publication_file/4/ijpp-10-3-001.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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