NDLI: The isoepoxydon dehydrogenase gene of the patulin metabolic pathway differs for Penicillium griseofulvum and Penicillium expansum

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The isoepoxydon dehydrogenase gene of the patulin metabolic pathway differs for Penicillium griseofulvum and Penicillium expansum

Content Provider	Semantic Scholar
Author	Dombrink-Kurtzman, Mary Ann
Copyright Year	2005
Abstract	Purified DNA from isolates of Penicillium griseofulvum and P. expansum was used as a template to amplify a 600-bp fragment of the isoepoxydon dehydrogenase (idh) gene of the patulin biosynthetic pathway. Primer pairs designed from the P. griseofulvum gene (GenBank accession AF006680) to amplify specific regions of the idh gene yielded similar-sized bands for all strains. Asymmetrical amplification produced DNA products for sequencing and DNA sequences were translated to produce the corresponding amino acid sequences. After removal of two introns present in the region sequenced, amino acid sequences were compared. There were 12 amino acid differences between P. expansum and P. griseofulvum in the coding region. The differences correlated with the amount of patulin previously produced in culture, with strains of P. griseofulvum producing the greatest amounts of patulin.
Starting Page	1
Ending Page	8
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1007/s10482-005-9002-5
PubMed reference number	16328863
Journal	Medline
Volume Number	89
Alternate Webpage(s)	https://naldc.nal.usda.gov/download/287/PDF
Alternate Webpage(s)	https://doi.org/10.1007/s10482-005-9002-5
Journal	Antonie van Leeuwenhoek
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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