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Something old, something new : exploring membrane-containing bacteriophages
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mäntynen, Sari |
| Copyright Year | 2016 |
| Abstract | Mäntynen, Sari Something old, something new Exploring membrane-containing bacteriophages Jyväskylä: University of Jyväskylä, 2016, 111 p. (Jyväskylä Studies in Biological and Environmental Science ISSN 1456-9701; 312) ISBN 978-951-39-6460-3 (nid.) ISBN 978-951-39-6461-0 (PDF) Yhteenveto: Jotain uutta, jotain vanhaa – kalvorakenteen sisältävät bakteriofagit Diss. Bacterial viruses, also called bacteriophages or phages, form a remarkably large and diverse group of biological entities. Bacteriophage studies have traditionally centered on tailed non-enveloped species, but a multitude of other virus types is gradually coming to light. Phages with a structural membrane component vary in terms of morphologies, genome types and replication mechanisms. Possibly the most comprehensively characterized membranecontaining phage is enterobacteria infecting PRD1 of the Tectiviridae family. However, there are still unanswered questions concerning its assembly process. This thesis adds new pieces to the puzzle by demonstrating that the subcellular distribution of fluorescently labelled PRD1 proteins is asymmetric, possibly resulting from spatially organized phage protein oligomerization and/or virion assembly. Moreover, PRD1 non-structural proteins P17 and P33 are shown to complement a defect in host co-chaperonin, suggesting a degree of functional redundancy between the viral and bacterial proteins. This thesis also introduces two novel bacteriophages: NN and FLiP infecting Pseudomonas and Flavobacterium species, respectively. The virion of NN contains a tri-segmented dsRNA genome enclosed by an icosahedral protein core and an outermost membrane envelope. Structural and genetic similarities strongly suggest that NN is a new member of the Cystoviridae family. Genetic and structural comparisons among the putative cystoviruses display the conservation of viral “self” components, essential for the formation of functional virions, while the sequences required for host recognition differ. On the other hand, the virion of FLiP consists of an icosahedral protein shell with an internal membrane, uniquely combined with a circular ssDNA genome. FLiP lacks any significant sequence homology to known bacteriophages, but its overall virion structure resembles strikingly that of dsDNA phage PM2, tentatively suggesting a relationship between these phages with different genome types. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://jyx.jyu.fi/bitstream/handle/123456789/48289/978-951-39-6461-0_vaitos15012015.pdf?sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
