NDLI: Controlling the Assembly of Coiled

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Controlling the Assembly of Coiled–Coil Peptide Nanotubes

Content Provider	Semantic Scholar
Author	Thomas, Franziska Burgess, Natasha C. Thomson, Andrew R. Woolfson, Derek N.
Copyright Year	2016
Abstract	An ability to control the assembly of peptide nanotubes (PNTs) would provide biomaterials for applications in nanotechnology and synthetic biology. Recently, we presented a modular design for PNTs using α-helical barrels with tunable internal cavities as building blocks. These first-generation designs thicken beyond single PNTs. Herein we describe strategies for controlling this lateral association, and also for the longitudinal assembly. We show that PNT thickening is pH sensitive, and can be reversed under acidic conditions. Based on this, repulsive charge interactions are engineered into the building blocks leading to the assembly of single PNTs at neutral pH. The building blocks are modified further to produce covalently linked PNTs via native chemical ligation, rendering ca. 100 nm-long nanotubes. Finally, we show that small molecules can be sequestered within the interior lumens of single PNTs.
Starting Page	987
Ending Page	991
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1002/anie.201509304
PubMed reference number	26663438
Journal	Medline
Volume Number	55
Alternate Webpage(s)	https://research-information.bristol.ac.uk/files/64217193/Thomas_et_al_2016_Angewandte_Chemie_International_Edition.pdf
Alternate Webpage(s)	http://eprints.gla.ac.uk/132135/1/132135.pdf
Alternate Webpage(s)	https://research-information.bris.ac.uk/files/64217193/Thomas_et_al_2016_Angewandte_Chemie_International_Edition.pdf
Journal	Angewandte Chemie
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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