Location of Geminiviruses in the Whitefly Bemisia tabaci (Homoptera: Aleyrodidae).

Content Provider	Semantic Scholar
Author	Hunter, Wayne B. Hiebert, Ernest Webb, Susan E. Tsai, Jennifer Polston, Jane E.
Copyright Year	1998
Abstract	The location of tomato mottle virus (ToMoV) and cabbage leaf curl virus (CabLCV) (Geminiviridae, genus Begomovirus) in the whitefly vector Bemisia tabaci B-biotype (Homoptera: Aleyrodidae) was elucidated using a novel technique incorporating indirect immunofluorescent labeling in freshly dissected whiteflies. Begomoviruses were visualized in the whitefly by indirect-fluorescent-microscopy. Polyclonal and monoclonal primary antibodies were used to successfully detect both ToMoV and CabLCV. Both begomoviruses were located in the anterior region of the midgut and filter-chamber of adult whiteflies, with ToMoV detected in the salivary glands. CabLCV was detected at a greater frequency than ToMoV, with a positive detection of 16% (89 out of 560) for CabLCV and 3% (25 out of 840) for ToMoV. Possible sites involved in geminivirus transport from the gut lumen of whiteflies into the hemocoel were located in the filter-chamber and anterior portion of the midgut. The location of these begomoviruses suggests a possible scenario of virus movement through the whitefly, which is discussed.
Starting Page	1147
Ending Page	1151
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1094/PDIS.1998.82.10.1147
Alternate Webpage(s)	https://www.ars.usda.gov/ARSUserFiles/60340505/1998%20Hunter%20et%20al%20Locate%20virus%20whitefly.pdf
PubMed reference number	30856777
Alternate Webpage(s)	https://doi.org/10.1094/PDIS.1998.82.10.1147
Journal	Medline
Volume Number	82
Issue Number	10
Journal	Plant disease
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article