NDLI: Hyperglycemia increases endothelial superoxide that impairs smooth muscle cell Na+-K+-ATPase activity.

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Hyperglycemia increases endothelial superoxide that impairs smooth muscle cell Na+-K+-ATPase activity.

Content Provider	Semantic Scholar
Author	Gupta, Sandeep Chough, Eugene Daley, Jennifer Oates, Peter J. Tornheim, Keith Ruderman, Neil B. Keaney, John F.
Copyright Year	2002
Abstract	Nitric oxide (NO) plays an important role in the control of numerous vascular functions including basal Na+-K+-ATPase activity in arterial tissue. Hyperglycemia inhibits Na+-K+-ATPase activity in rabbit aorta, in part, through diminished bioactivity of NO. The precise mechanism(s) for such observations, however, are not yet clear. The purpose of this study was to examine the role of superoxide in modulating NO-mediated control of Na+-K+-ATPase in response to hyperglycemia. Rabbit aorta incubated with hyperglycemic glucose concentrations (44 mM) demonstrated a 50% reduction in Na+-K+-ATPase activity that was abrogated by superoxide dismutase. Hyperglycemia also produced a 50% increase in steady-state vascular superoxide measured by lucigenin-enhanced chemiluminescence that was closely associated with reduced Na+-K+-ATPase activity. Specifically, the hyperglycemia-induced increase in vascular superoxide was endothelium dependent, inhibited by L-arginine, and stimulated by N(omega)-nitro-L-arginine. Aldose reductase inhibition with zopolrestat also inhibited the hyperglycemia-induced increase in vascular superoxide. In each manipulation of vascular superoxide, a reciprocal change in Na+-K+-ATPase activity was observed. Finally, a commercially available preparation of Na+-K+-ATPase was inhibited by pyrogallol, a superoxide generator. These data suggest that hyperglycemia induces an increase in endothelial superoxide that inhibits the stimulatory effect of NO on vascular Na+-K+-ATPase activity.
Starting Page	669
Ending Page	675
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1152/ajpcell.00343.2001
PubMed reference number	11832341
Journal	Medline
Volume Number	282
Issue Number	3
Alternate Webpage(s)	http://ajpcell.physiology.org/content/ajpcell/282/3/C560.full.pdf
Alternate Webpage(s)	https://doi.org/10.1152/ajpcell.00343.2001
Journal	American journal of physiology. Cell physiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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