NDLI: High cell density culture of Anabaena variabilis with controlled light intensity and nutrient supply.

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High cell density culture of Anabaena variabilis with controlled light intensity and nutrient supply.

Content Provider	Semantic Scholar
Author	Yoon, Jong Hyun Shin, Jong-Hwan Ahn, Eun Kyung Park, Tai Hyun
Copyright Year	2008
Abstract	Controlling the light energy and major nutrients is important for high cell density culture of cyanobacterial cells. The growth phase of Anabaena variabilis can be divided into an exponential growth phase and a deceleration phase. In this study, the cell growth in the deceleration phase showed a linear growth pattern. Both the period of the exponential growth phase and the average cell growth rate in the deceleration phase increased by controlling the light intensity. To control the light intensity, the specific irradiation rate was maintained above 10 micromol/s/g dry cell by increasing the incident light intensity stepwise. The final cell density increased by controlling the nutrient supply. For the control of the nutrient supply, nitrate, phosphate, and sulfate were intermittently added based on the growth yield, along with the combined control of light intensity and nutrient concentration. Under these control conditions, both final cell concentration and cell productivity increased, to 8.2 g/l and 1.9 g/l/day, respectively.
File Format	PDF HTM / HTML
PubMed reference number	18633292
Journal	Medline
Volume Number	18
Issue Number	5
Alternate Webpage(s)	http://s-space.snu.ac.kr/bitstream/10371/9526/1/2008.05_JMB_High%20Cell%20Density.pdf
Journal	Journal of microbiology and biotechnology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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