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Measurement and restoration of the point spread function of fluorescence confocal microscopy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yoo, Herbert Song, Incheon Gweon, D-G |
| Copyright Year | 2006 |
| Abstract | The point spread function of an objective lens of a fluorescence confocal microscope was directly measured by imaging fluorescent beads. We analysed how the measurement of the point spread function was influenced by the diameter of the fluorescent beads and how the restoration technique with a deconvolution algorithm improved the measuring performance. Numerical and experimental results are presented for a typical point spread function and a zero-centred point spread function. |
| File Format | PDF HTM / HTML |
| DOI | 10.1111/j.1365-2818.2006.01556.x |
| PubMed reference number | 16551278 |
| Journal | Medline |
| Volume Number | 221 |
| Part | 3 |
| Alternate Webpage(s) | http://koasas.kaist.ac.kr/bitstream/10203/16594/1/%5B2006%5DMeasurement%20and%20Restoration%20of%20the%20Point%20Spread%20Function%20of%20Fluorescence%20Confocal%20Microscopy.pdf |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1365-2818.2006.01556.x |
| Journal | Journal of microscopy |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |