NDLI: Twenty-five new polymorphic microsatellites for the eastern mosquitofish, Gambusia holbrooki (Actinopterygii : Poeciliidae), an invasive species in Australia

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Twenty-five new polymorphic microsatellites for the eastern mosquitofish, Gambusia holbrooki (Actinopterygii : Poeciliidae), an invasive species in Australia

Content Provider	Semantic Scholar
Author	Umbers, Kate D. L. Jennions, Michael D. Gardner, Michael G. Keogh, J. Scott
Copyright Year	2012
Abstract	Abstract. We isolated 25 new polymorphic microsatellite markers from the eastern mosquitofish, Gambusia holbrooki. Initially, 454 shotgun sequencing was used to identify 1187 loci for which primers could be designed. Of these 1187, we trialled 48 in the target species, 40 of which amplified a product of expected size. Subsequently, those 40 loci were screened for variation in 48 individuals from a single population in Canberra, Australia. Twenty loci were in Hardy–Weinberg equilibrium and polymorphic, with observed heterozygosity ranging from 0.04 to 0.72 (mean: 0.45 ± 0.18) and the number of alleles per locus ranged from 2 to 5 (mean: 3.20 ± 1.05). These loci will be useful in understanding genetic variation, paternity analysis and in managing this species across both its native and invasive range.
Starting Page	235
Ending Page	237
Page Count	3
File Format	PDF HTM / HTML
DOI	10.1071/ZO12095
Alternate Webpage(s)	http://thejennionslab.weebly.com/uploads/2/6/5/0/26509069/paper140.pdf
Alternate Webpage(s)	https://doi.org/10.1071/ZO12095
Volume Number	60
Journal	Australian Journal of Zoology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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