NDLI: High-performance liquid chromatography (HPLC) determination of five active ingredients in the calyces of Physalis Alkekengi L. var. franchetii (mast.) Mskino

Content Provider	Semantic Scholar
Author	Xu, Baoli Ju, Chengguo Guan, Huijie Xu, Liang Wang, Bing
Copyright Year	2012
Abstract	A simple high-performance liquid chromatographic (HPLC) assay using the internal standard method is developed for the simultaneous determination of five active ingredients. The analyzed compounds from the calyces of Physalis alkekengi L. var. franchetii (mast.) Mskino include Luteoloside, Luteolin, Physalin A, Physalin O and Physalin P. HPLC analysis is performed on an Agilent C18 analytical column (150×4.6 mm and 5 μm) using solvent (A) acetonitrile and (B) 0.2% aqueous phosphoric acid as the mobile phase with Ultraviolet (UV) absorption at 350 and 220 nm. The flow rate was 1.0 ml/min. The calibration curves of the five active ingredients are linear (r 2 > 0.9991) over the concentration range of 10 to 120 μg/ml. The mean recoveries were 97.59 to 101.04%. The results indicate that the HPLC method developed can easily be applied to the determination of five active ingredients in the calyces of P. alkekengi L. var. franchetii (mast.) Mskino.
File Format	PDF HTM / HTML
DOI	10.5897/JMPR11.1435
Volume Number	6
Alternate Webpage(s)	https://academicjournals.org/journal/JMPR/article-full-text-pdf/4BC4D2734763
Alternate Webpage(s)	https://doi.org/10.5897/JMPR11.1435
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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