NDLI: Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells.

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Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells.

Content Provider	Semantic Scholar
Author	Li, Rui-Dong Deng, Zhong-Liang Hu, Ning Liang, Xi Luo, Jinyong Chen, Liang Yin, Liang-Jun Luo, Xiaoji Shui, Wei He, Tong-Chuan Huang, Wei
Copyright Year	2012
Abstract	We have found that the previously uncharacterized bone morphogenetic protein-9 (BMP9) is one of the most osteogenic factors. However, it is unclear if BMP9 cross-talks with TGFβ1 during osteogenic differentiation. Using the recombinant BMP9 adenovirus, we find that low concentration of rhTGFβ1 synergistically induces alkaline phosphatase activity in BMP9-transduced C3H10T1/2 cells and produces more pronounced matrix mineralization. However, higher concentrations of TGFβ1 inhibit BMP9-induced osteogenic activity. Real-time PCR and Western blotting indicate that BMP9 in combination with low dose of TGFβ1 potentiates the expression of later osteogenic markers osteopontin, osteocalcin and collagen type 1 (COL1a2), while higher concentrations of TGFβ1 decrease the expression of osteopontin and osteocalcin but not COL1a2. Cell cycle analysis reveals that TGFβ1 inhibits C3H10T1/2 proliferation in BMP9-induced osteogenesis and restricts the cells in G(0)/G(1) phase. Our findings strongly suggest that TGFβ1 may exert a biphasic effect on BMP9-induced osteogenic differentiation of mesenchymal stem cells.
File Format	PDF HTM / HTML
DOI	10.5483/BMBRep.2012.45.9.053
PubMed reference number	23010171
Journal	Medline
Volume Number	45
Issue Number	9
Journal	BMB reports
Alternate Webpage(s)	http://ocean.kisti.re.kr/downfile/volume/ksbmb/E1MBB7/2012/v45n9/E1MBB7_2012_v45n9_509.pdf
Alternate Webpage(s)	http://www.boneandcancer.org/MOLab%20Publications%20in%20PDF%20files/Li%20RD%20et%20al_BMB%2012-053%20proof.pdf
Alternate Webpage(s)	https://doi.org/10.5483/BMBRep.2012.45.9.053
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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