NDLI: Evaluierung von Biomarkern im Liquor cerebrospinalis bei paraplegischen Hunden mit Rückenmarksverletzung

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Evaluierung von Biomarkern im Liquor cerebrospinalis bei paraplegischen Hunden mit Rückenmarksverletzung

Content Provider	Semantic Scholar
Author	Wicha, Stefanie I.
Copyright Year	2016
Abstract	Intervertebral disc herniation is a common cause of spinal cord injuries (SCI) in dogs. The prognosis in these dogs is determined by the severity of SCI and the degree of neurological deficits. The prediction of a reliable prognosis can be challenging in paraplegic dogs, particularly when they lost nociception. Tau protein, macrophage inflammatory protein-3s (MIP-3s) and glial fibrillary acidic protein (GFAP) are potential markers of SCI. The aim of this study was to assess the value of these substances as cerebrospinal fluid (CSF) biomarkers for dogs with SCI. Therefore, the potential of these markers to quantify the degree of spinal cord damage was evaluated and whether they could consequently serve as prognostic indicators. Additionally, a calculation of the combination of multiple CSF biomarkers was performed in order to assess whether this could enhance the predictive value. The concentrations of tau protein, MIP-3s and GFAP were measured in cisternal and lumbar CSF samples of 49 paraplegic dogs using enzyme-linked immunosorbent assays (ELISA). The dogs with acute/subacute ( 28 days; n = 13) SCI were classified according to the presence (grade 4) or absence (grade 5) of deep pain perception (Sharp & Wheeler, 2005). Outcome of dogs with acute/subacute SCI was monitored by neurological follow-up exams and was defined to be successful when an improvement of at least one grade was noted within four weeks after surgery. The CSF of seven healthy beagle dogs served as control. Tau protein and MIP-3s concentrations were significantly higher in paraplegic dogs with acute/subacute SCI compared to healthy dogs and to dogs with chronic SCI. GFAP concentration in cisternal CSF of dogs with acute/subacute SCI and grade 5 were significantly higher compared to healthy dogs. However, no difference in GFAP concentrations between paraplegic and healthy dogs was detected in lumbar CSF nor between dogs with acute/subacute and chronic SCI. Paraplegic dogs with chronic SCI had significantly elevated tau protein and MIP-3s concentrations in lumbar CSF compared to control dogs. Dogs with neurological improvement had significantly lower cisternal tau protein values, especially those without deep pain perception. This predictive value of CSF tau protein concentrations could not be enhanced by a combination of multiple CSF biomarkers. In conclusion, tau protein, MIP-3s and GFAP are potential biomarkers in dogs with SCI. The cisternal tau protein concentration may serve as a prognostic indicator in dogs with SCI. A combination of all three CSF biomarkers together could not enhance the prognostic accuracy.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://elib.tiho-hannover.de/servlets/MCRFileNodeServlet/etd_derivate_00000288/wichas_ss16.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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