CD4+ T cell responses in mice lacking MHC class II molecules specifically on B cells.

Content Provider	Semantic Scholar
Author	Williams, Geoffrey S. Oxenius, Annette Hengartner, Hans Mathis, Diane
Copyright Year	1998
Abstract	The role of B lymphocytes in initiating and maintaining a CD4+ T cell response has been examined using a variety of strategies, but remains controversial because of weaknesses inherent to each of the approaches. Here, we address this issue by measuring CD4+ T cell priming both in mutant mice devoid of B cells and in chimeric animals lacking major histocompatibility complex class II molecules specifically on B cells. We find that peptide and some protein antigens do not require B cells expressing class II molecules, nor B cells themselves, to efficiently prime. This could be demonstrated by the usual lymph node proliferation assay, a rather indirect in vitro measure of priming, and by a direct ex vivo assay of population expansion and activation marker expression. Interestingly, one protein antigen, conalbumin, could not prime in the absence of B cells, but could in the presence of B cells devoid of class II molecules. This finding constrains the possible mechanisms whereby B lymphocytes contribute to the initiation of a CD4+ T cell response, arguing against the importance of surface immunoglobulin-mediated antigen presentation by B cells.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://cbdm.hms.harvard.edu/assets/Publications/1998pub/Williams.pdf
PubMed reference number	9842919v1
Volume Number	28
Issue Number	11
Journal	European journal of immunology
Language	English
Access Restriction	Open
Subject Keyword	Anatomic Node Antigen Presentation B-Lymphocytes Chimera organism Conalbumin Leukemia, B-Cell Lymph Node Tissue Major Histocompatibility Complex Priming Exercise Receptors, Antigen, B-Cell T-Lymphocyte Transcription Initiation Weakness lymph nodes
Content Type	Text
Resource Type	Article