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4827-quadruplex for DNA detection : new insight into the formation of DNA split G-quadruplex †
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhu, Jinbo Zhang, Libing Donga, Shaojun Wang, Erkang |
| Copyright Year | 2015 |
| Abstract | Here, we get a new insight into the formation of a split G-quadruplex from the viewpoints of the split mode and guanine base number. An unusual result is that the split mode 4 : 8 performed best in six split modes, including the frequently used mode 1 : 3 and 2 : 2 in the split G-quadruplex enhanced fluorescence assay. Circular dichroism spectra verified the conclusion. The application of the split G-quadruplex based assay in DNA detection was performed on the point mutations of the JAK2 V617F and HBB genes. A multi-target analysis method based on a pool of G-segments split from T30695 (GGGTGGGTGGGTGGGT) by the magic “law of 4 : 8” was established. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://pubs.rsc.org/en/content/articlepdf/2015/sc/c5sc01287b |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
