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A Digital Scanned Laser Light Sheet Microscope (dslm)
Content Provider | Semantic Scholar |
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Author | Keller, Philipp J. Stelzer, Ernst H. K. Heidelberg, Embl |
Copyright Year | 2007 |
Abstract | Light sheet microscopy, fluorescence microscopy, 3D imaging, structured illumination, SPIM, high-throughput. Single Plane Illumination Microscopy (SPIM) provides fast three-dimensional recording of fluorescent biological specimens [1]. Photobleaching and phototoxic effects are minimized since only the fluorophores in the common volume of the illumination light sheet and the focal plane of the detection lens are excited. These features (amongst many others) make SPIM valuable in studies of fast biological processes at high magnification as well as for |
File Format | PDF HTM / HTML |
Alternate Webpage(s) | http://www.focusonmicroscopy.org/2007/PDF/267_Keller.pdf |
Language | English |
Access Restriction | Open |
Content Type | Text |
Resource Type | Article |