NDLI: AFLP Analysis and Improved Phytoextraction Capacity of Transgenic gshI-Poplar Clones (Populus x canescens L.) for Copper in vitro

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AFLP Analysis and Improved Phytoextraction Capacity of Transgenic gshI-Poplar Clones (Populus x canescens L.) for Copper in vitro

Content Provider	Semantic Scholar
Author	Gyulai, Gâbor Humphreys, Mervyn W. Bittsánszky, András Skøtc, Kirsten Kiss, József Károly Skøt, Leif Gullner, Gábor Heywood, Sue Szabó, Zoltán Lovatt, Alan Radimszky, László Roderick, H. W. Rennenberg, Heinz Abberton, Michael T. Kőmívesd, Tamas Heszky, László E.
Copyright Year	2005
Abstract	Abstract Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRIDMseI digestion. Four fragments generated by EcoAGTDMseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. \ canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10-1 to 10-5 ᴍ) incubated for 21 days on aseptic tissue culture media WPM containing 1 μᴍ Cu. Zn2+ caused phytotoxicity only at high concentrations (10-1 to 10-2 ᴍ). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.
Starting Page	300
Ending Page	306
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1515/znc-2005-3-415
Alternate Webpage(s)	https://www.degruyter.com/downloadpdf/j/znc.2005.60.issue-3-4/znc-2005-3-415/znc-2005-3-415.pdf
Alternate Webpage(s)	http://www.znaturforsch.com/ac/v60c/60c0300.pdf
Alternate Webpage(s)	http://www.znaturforsch.com/ac/v60c/s60c0300.pdf
Alternate Webpage(s)	http://real.mtak.hu/5492/1/1145805.pdf
PubMed reference number	15948599
Alternate Webpage(s)	https://doi.org/10.1515/znc-2005-3-415
Volume Number	60
Journal	Zeitschrift fur Naturforschung. C, Journal of biosciences
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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