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Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ryder, Edward Doe, Brendan Gleeson, Diane Houghton, Richard Dalvi, Priya Grau, E. Habib, Bishoy Miklejewska, Evelina Newman, Stuart Sethi, Debarati Sinclair, Caroline Louise Vyas, Sapna Wardle-Jones, Hannah Bottomley, Joanna Bussell, James N. Galli, Antonella Salisbury, Jennifer Solís, Ramiro Ramírez‐ |
| Copyright Year | 2013 |
| Abstract | We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits. |
| Starting Page | 177 |
| Ending Page | 185 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11248-013-9764-x |
| PubMed reference number | 24197666 |
| Journal | Medline |
| Volume Number | 23 |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/30/3f/11248_2013_Article_9764.PMC3890051.pdf |
| Journal | Transgenic Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |